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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
28/08/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
VÁSQUEZ-GARCÍA, A.; SILVA, T. dos S.; ALMEDA-QUEIROZ, S. R. de; GODOY, S. H. S.; FERNANDES, A. M.; SOUSA, R. L. M.; FRANZOLIN, R. |
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Afiliação: |
Andrea Vásquez-García, Programa de Pós-Graduação em Ciências, Departamento de Engenharia de Alimentos/Faculdade de Zootecnia e Engenharia de Alimentos - FZEA/Universidade de São Paulo - USP; Thaysa dos Santos Silva, Programa de Pós-Graduação em Zootecnia / Departamento de Zootecnia - FZEA / USP; Sabrina R. de Almeida-Queiroz, Departamento de Zootecnia e Medicina Veterinária - FZEA / USP; Silvia H. S. Godoy, Departamento de Zootecnia e Medicina Veterinária - FZEA / USP; Andrezza M. Fernandes, Departamento de Zootecnia e Medicina Veterinária - FZEA / USP; Ricardo L. M. Sousa, Departamento de Zootecnia e Medicina Veterinária - FZEA / USP; Raul Franzolin, Departamento de Zootecnia - FZEA / USP. |
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Título: |
Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 5, p. 447-452, maio 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Identificação de espécies e perfil de susceptibilidade antimicrobiano de bactérias causadoras de mastite subclínica em búfalos. |
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Conteúdo: |
Microorganisms causing subclinical mastitis in water buffalo were isolated from 20 buffalo milk samples at four dairy farms located in central region of São Paulo State, Brazil, through testing of somatic cell count (SCC), standard plate count (SPC), biochemical, PCR assays and antimicrobial profile. The SCC showed average of 721,000 cells/mL in the milk, indicating the presence of subclinical mastitis. The overall average for SPC was 1.8 x 104 CFU/mL. The microorganism most frequently isolation according to biochemical tests were: Staphylococcus epidermidis (17%), Staphylococcus aureus (15%), Bacillus spp. (14%), Acinetobacter spp. (12.5%); with intermediate frequency: Pseudomonas aeruginosa (9.5%); Shigella flexneri (7.0%), Streptococcus spp. (5.5%), Corynebacterium spp. (5.0%), Escherichia coli (4.5%), Serratia marcescens (4.0%), Stenotrophomonas maltophilia (4.0%), and low incidence: Klebsiella rhinoscleromatis (0.5%), Klebsiella ozaenae (0.5%), Tatumella ptyseos (0.5%), Enterobacter cloacae (0.5%). The molecular analysis indicated that samples positive by culture method of the genera Staphylococcus, Streptococcus and E. coli were positive by PCR. Para S. aureus and S. epidermidis the highest percentages of observed sensitivity were gentamicin (100%) and vancomycin (100%); for the genus Streptococcus to gentamicin and oxacillin and E. coli to Ampicilin. These findings may help in the control and treatment of subclinical mastitis in buffaloes and contribute to improving the efficiency and quality of the milk produced. MenosMicroorganisms causing subclinical mastitis in water buffalo were isolated from 20 buffalo milk samples at four dairy farms located in central region of São Paulo State, Brazil, through testing of somatic cell count (SCC), standard plate count (SPC), biochemical, PCR assays and antimicrobial profile. The SCC showed average of 721,000 cells/mL in the milk, indicating the presence of subclinical mastitis. The overall average for SPC was 1.8 x 104 CFU/mL. The microorganism most frequently isolation according to biochemical tests were: Staphylococcus epidermidis (17%), Staphylococcus aureus (15%), Bacillus spp. (14%), Acinetobacter spp. (12.5%); with intermediate frequency: Pseudomonas aeruginosa (9.5%); Shigella flexneri (7.0%), Streptococcus spp. (5.5%), Corynebacterium spp. (5.0%), Escherichia coli (4.5%), Serratia marcescens (4.0%), Stenotrophomonas maltophilia (4.0%), and low incidence: Klebsiella rhinoscleromatis (0.5%), Klebsiella ozaenae (0.5%), Tatumella ptyseos (0.5%), Enterobacter cloacae (0.5%). The molecular analysis indicated that samples positive by culture method of the genera Staphylococcus, Streptococcus and E. coli were positive by PCR. Para S. aureus and S. epidermidis the highest percentages of observed sensitivity were gentamicin (100%) and vancomycin (100%); for the genus Streptococcus to gentamicin and oxacillin and E. coli to Ampicilin. These findings may help in the control and treatment of subclinical mastitis in buffaloes and contribute to improving t... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial profile; Buffalo; Contagem de células somáticas; Mastite subclínica; Susceptibilidade antimicrobiano. |
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Thesagro: |
Bactéria; Búfalo; Leite. |
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Thesaurus Nal: |
Antibiotics; mastitis; Milk; Somatic cell count. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163092/1/Species-identification-and-antimicrobial.pdf
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Marc: |
LEADER 02751naa a2200349 a 4500
001 2074556
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aVÁSQUEZ-GARCÍA, A.
245 $aSpecies identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Identificação de espécies e perfil de susceptibilidade antimicrobiano de bactérias causadoras de mastite subclínica em búfalos.
520 $aMicroorganisms causing subclinical mastitis in water buffalo were isolated from 20 buffalo milk samples at four dairy farms located in central region of São Paulo State, Brazil, through testing of somatic cell count (SCC), standard plate count (SPC), biochemical, PCR assays and antimicrobial profile. The SCC showed average of 721,000 cells/mL in the milk, indicating the presence of subclinical mastitis. The overall average for SPC was 1.8 x 104 CFU/mL. The microorganism most frequently isolation according to biochemical tests were: Staphylococcus epidermidis (17%), Staphylococcus aureus (15%), Bacillus spp. (14%), Acinetobacter spp. (12.5%); with intermediate frequency: Pseudomonas aeruginosa (9.5%); Shigella flexneri (7.0%), Streptococcus spp. (5.5%), Corynebacterium spp. (5.0%), Escherichia coli (4.5%), Serratia marcescens (4.0%), Stenotrophomonas maltophilia (4.0%), and low incidence: Klebsiella rhinoscleromatis (0.5%), Klebsiella ozaenae (0.5%), Tatumella ptyseos (0.5%), Enterobacter cloacae (0.5%). The molecular analysis indicated that samples positive by culture method of the genera Staphylococcus, Streptococcus and E. coli were positive by PCR. Para S. aureus and S. epidermidis the highest percentages of observed sensitivity were gentamicin (100%) and vancomycin (100%); for the genus Streptococcus to gentamicin and oxacillin and E. coli to Ampicilin. These findings may help in the control and treatment of subclinical mastitis in buffaloes and contribute to improving the efficiency and quality of the milk produced.
650 $aAntibiotics
650 $amastitis
650 $aMilk
650 $aSomatic cell count
650 $aBactéria
650 $aBúfalo
650 $aLeite
653 $aAntimicrobial profile
653 $aBuffalo
653 $aContagem de células somáticas
653 $aMastite subclínica
653 $aSusceptibilidade antimicrobiano
700 1 $aSILVA, T. dos S.
700 1 $aALMEDA-QUEIROZ, S. R. de
700 1 $aGODOY, S. H. S.
700 1 $aFERNANDES, A. M.
700 1 $aSOUSA, R. L. M.
700 1 $aFRANZOLIN, R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 5, p. 447-452, maio 2017.
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Embrapa Unidades Centrais (AI-SEDE) |
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